تولید داربست های نانولیفی پوسته- مغزی رسانا جهت بهبود ترمیم ضایعه نخاعی

نوع مقاله: مقاله پژوهشی

نویسندگان

1 استادیار، دانشگاه حضرت معصومهسلام الله علیها، قم

2 استاد، دانشکده مهندسی نساجی، دانشگاه صنعتی امیرکبیر، تهران

3 استادیار، گروه آناتومی، دانشکده پزشکی، دانشگاه علوم پزشکی گیلان، رشت، ایران

چکیده

از آنجا که درمان ضایعات نخاعی به علت پیچیدگی سیستم اعصاب مرکزی به سادگی امکان پذیر نیست، راه کارهای بازسازی و ترمیم بافت عصب در دنیا بسیار مورد توجه واقع شده است. در این پژوهش دو نوع داربست نانولیفی سه بعدی رسانا (پلی لاکتیک گلایکولیک اسید- پلی کاپرولاکتان/پلی آنیلین: PCL/PANI-PLGA) و نارسانا (پلی لاکتیک گلایکولیک اسید- پلی لاکتیک گلایکولیک اسید PLGA-PLGA) با ساختار پوسته- مغزی طراحی و توسط یک شیوه الکتروریسی ترکیبی تولید شده است. سامانه الکتروریسی ترکیبی طراحی شده دارای یک سامانه گردابی و یک سامانه دو نازله بوده و قادر به تولید داربست استوانه ای سه بعدی با پوسته زبر نانومقیاس و مغزی آرایش یافته (میکرورشته های آرایش یافته شامل نانوالیاف آرایش یافته) می باشد. بر اساس آزمایش ها و بررسی ها در شرایط برون تنی و درون تنی، بیشترین میزان تکثیر سلول های عصبی بر روی داربست سه بعدی با مغزی رسانا مشاهده گردید. در بررسی های حیوانی نیز نتایج آزمایش حرکتی بیانگر بهبود معنادار نمونه های پیوند زده شده نسبت به نمونه های شاهد می باشد.

کلیدواژه‌ها

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