A novel nano multi MIP sensor for simultaneous detection of cTnT & HbA1c

Document Type : Research Article

Authors

1 Biomaterials Group, Faculty of Biomedical Engineering, AmirKabir University of Technology, Tehran, Iran

2 Biomaterials Group, Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran

3 Department of Developmental Sciences, Marquette University, Milwaukee, WI 53233, USA

4 Assistant Professor, Maziar University, Tehran, Iran

/amnc.2019.8.30.7

Abstract

A nano-molecularly imprinted polymer (N-MIP) dual-sensor based on a graphene screen-printed electrode was developed for the detection of glycated hemoglobin and cardiac troponin T (cTnT), separately. In order to obtain a biomimetic surface, a conductive co-polymer matrix was deposited on the surface of graphene oxide (GO) electrode. The sensor probe was modified via electropolymerization of aniline and carboxylated aniline on the graphene oxide (GO) electrode, in the presence template proteins (cTnT for cardiac troponin T probe and HbA1c for glycated hemoglobin probe) by cyclic voltammetry.

The surfaces of both sensors were characterized using cyclic and differential pulse voltammetry (CV), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), electrochemical impedance spectroscopy (EIS), quartz crystal microbalance (QCM). The best biomimetic surface nanotexture was obtained at aniline/carboxylatedaniline ratio of 1:4. The linear ranges of cTnT and HbA1c were from 0.02 to 0.09 ng/mL and from 0.004 to 0.81 μg/mL, with detection limits of 0.008 ng/mL and 4.3 ng/mL, respectively. The reliability of the M-NIP cTnT and HbA1c sensors was examined by comparing the results with those obtained from HPLC method and It was observed that the results from N-MIP sensors and HPLC had a great correlation.

Keywords

Main Subjects


 
[1] A.Maqsood, K. A. Kaid, M. Cohen, Int. J. Cardiovas. Res., 2007, 4:1-5.
[2] B. Cummins, M. L. Auckland and P. Cummins, Cardiac-specific troponin-I radioimmunoassay in the diagnosis of acute myocardial infarction. Am. Heart J. (1987), 113:1333-44.
[3] K. Matsuoka, M. Maeda, A. Tsuji, Fluorescence enzyme immunoassay for insulin using peroxidase-tyramine-hydrogen peroxide. Chem. Pharm. Bull. (1979), 27:2345-50.
[4] P. Norouzi, B. Larijani, F. Faridbod, M. R. Ganjali , Hydrogen Peroxide Biosensor Based on Hemoglobin Immobilization on Gold Nanoparticle in FFT Continuous Cyclic Voltammetry Flow Injection System. Int. J. Electrochem. Sci. 5(2010), 1550 –62.
[5] A.J. S. Ahammad, S. Sarker and J.-J. Lee, J. Nanosci. Immobilization of Horseradish Peroxidase onto a Gold-Nanoparticle-Adsorbed Poly(thionine) Film for the Construction of a Hydrogen Peroxide Biosensor. Nanotechnol. 11(2011), 5670-76.
[6] A. J. Saleh Ahammad, Yo-Han Choi, Electrochemical Detection of Cardiac Biomarker Troponin I at Gold Nanoparticle-Modified ITO Electrode by Using Open Circuit Potential. Int. J. Electrochem. Sci. (2011), 1906 – 16.
[7] B. I. Podlovchenko, T. D. Gladysheva, E. A. Kolyadko, Experimental check-up of the relationship between transients of current and open circuit potential for strong adsorption of neutral species and ions on a hydrogen electrode. J. Electroanal. Chem. 552(2003), 85-96.
[8] J. P. Wilburn, M. Ciobanu, D. A. Lowy, Characterization of Acrylic Hydrogels by Open Circuit Potential Monitoring. J. Appl. Electrochem. 34(2004), 729-734.
[9] Bárbara V.M. Silva, Igor T, Disposable immunosensor for human cardiac troponin T based on streptavidin-microsphere modified screen-printed electrode. Biosensors and Bioelectronics. 26(2010), 1062–67.
[10] S. Ko, et al. , Electrochemical detection of cardiac troponin I using a microchip with the surface-functionalized poly(dimethylsiloxane) channel. Biosensors and Bioelectronics. 23(2007), 51–9.
[11] Allen B.L., Kichambare P.D., Star, A. Carbon nanotube field-effect-transistor-based biosensors. Adv. Mater. 19 (2007), 1439–51.
[12] Y. Liu, M. Wei, Y. Hu, L. Zhu, J. Du, An electrochemical sensor based on a molecularly imprinted polymer for determination of anticancer drug Mitoxantrone, Sens. Actuator B- Chem. 255(2018), 544-551.
[13] L. Uzun, A.P.F. Turner, Molecularly-imprinted polymer sensors: realising their potential. Biosens. Bioelectron. 76 (2016), 131-144.
[14] W. Guo, F. Pi, H. Zhang, J. Sun, Y. Zhang, X. Sun, A novel molecularly imprinted electrochemical sensor modified with carbon dots, chitosan, gold nanoparticles for the determination of patulin. Biosens. Bioelectron. 98 (2017), 299-304.
[15] Z.Z. Yin, S.W. Cheng, L.B. Xu, et al., Highly sensitive and selective sensor for sunset yellow based on molecularly imprinted polydopamine-coated multi-walled carbon nanotubes, Biosens. Bioelectron. 100 (2018), 565-570
[16] Jin Zhang, Chaoying Wang, Yanhui Niu, Shijie Li, Rongqin Luo, Electrochemical sensor based on molecularly imprinted composite membrane of poly(o-aminothiophenol) with gold nanoparticles for sensitive determination of herbicide simazine in environmental samples. Sens. Actuator B-Chem. 249 (2017), 747-755.
[17] J. Bai, X. Zhang, Y. Peng, X. Hong, Y. Liu, S. Jiang, Z. Gao, Ultrasensitive sensing of diethylstilbestrol based on AuNPs/MWCNTs-CS composites coupling with sol-gel molecularly imprinted polymer as a recognition element of an electrochemical sensor. Sens. Actuator B- Chem. 238 (2017), 420-426.
[18] M. Soleimani, Ma. Ghahraman Afshar, A. Shafaat, G.A. Crespo, High‐Selective Tramadol Sensor Based on Modified Molecularly Imprinted Polymer-Carbon Paste Electrode with Multiwalled Carbon Nanotubes. ELECTROANAL. 25 (2013), 1159–1168.
[19] Y. Sun, H. Du, Y. Lan, W. Wang, Y. Liang, C. Feng, M. Yang, Preparation of hemoglobin (Hb) imprinted polymer by Hb catalyzed eATRP and its application in biosensor. Biosens. Bioelectron. 77 (2016), 894-900.
[20] N. Karimian, M. Vagin, M.H. Arbab Zavar, M. Chamsaz, A.P.F. Turner, A. Tiwari, An ultrasensitive molecularly-imprinted human cardiac troponin sensor. Biosens. Bioelectron. 50 (2013), 492-498.
[21] F.T.C.Moreira, M.J.M.S. Ferreira, J.R.T. Puga, M.G.F. Sales, Screen-printed electrode produced by printed-circuit board technology. Application to cancer biomarker detection by means of plastic antibody as sensing material. Sens. Actuator B- Chem. 223 (2016), 927-935.
[22] M. Abdorahim, M. Rabiee, S. Naghavi Alhosseini, M.R. Tahriri, S. Yazdanpanah, S.H. Alavi, L. Tayebi, Nanomaterials-based electrochemical immunosensors for cardiac troponin recognition: An illustrated review. Trends Analyt. Chem. 82 (2016), 337-347.
[23] S.Yazdanpanah, M. Rabiee, M.R. Tahriri, M. Abdolrahim, L. Tayebi, Glycated hemoglobin-detection methods based on electrochemical biosensors. Trends Analyt. Chem. 72 (2015), 53-67.
[24] S. N. Alam, N. Sharma, L. Kumar, Synthesis of Graphene Oxide (GO) by Modified Hummers Method and Its Thermal Reduction to Obtain Reduced Graphene Oxide (rGO). Graphene, 6 (2017), 1-18.
[25] B. V. M. Silva , B. A. G. Rodríguez, G. F. Sales, M. D. P. T. Sotomayor, R. F. Dutra, An ultrasensitive human cardiac troponin T graphene screen-printed electrode based on electro polymerized-molecularly imprinted conducting polymer, Biosensors and Bioelectronics, 77 (2016), 978–985.
[26] O. Krupin, P. Berini, Long-Range Surface Plasmon-Polariton Waveguide Biosensors for Human Cardiac Troponin I Detection. Sensors (Basel). 3 (2019), 631
[27] H. Lin, J. Yi, Current Status of HbA1c Biosensors. Sensors (Basel). 3 (2017), 1798