Rapid and Easy Fabrication of Tryptophan Amino Acid Plasmonic Detection Kit

Document Type : Research Article

Authors

1 Nanoscience and Nanotechnology Research Center, University of Kashan, Kasahn, Iran

2 Physics Department, University of Kashan, Kasahn, Iran

/amnc.2020.9.33.1

Abstract

In this experimental study, silver colloidal solution was synthesized using Tollens method. Plasmonic kit was fabricated by drop-coating of silver colloidal solution on glass substrate and finally, detection of tryptophan amino acid was performed using plasmonic kit and surface enhanced Raman spectroscopy. The field emission scanning electron microscopy (FESEM) image shows the most of silver nanoparticles on plasmonic kit have the sizes between from 1400 to 1500 nm. Plasmon peak at around 410 and observation of FCC structure in XRD characterization confirmed the formation of silver nanoparticles. Surface plasmon resonance of silver nanoparticles as well as light scattering from larger agglomerated silver particles enhance the molecular vibrations of tryptophan amino acid. By calibrating the intensity of molecular vibrations in terms of tryptophan amino acid concentration, a quadratic relationship was obtained from which the concentration of the tryptophan amino acid could be determined by measuring SERS spectra. Raman spectroscopy improves the Raman signals of tryptophan amino acid due to the resonance of surface plasmons of silver nanoparticles and the light scattering from larger silver particles. As the tryptophan amino acid concentration decreases, the SERS signals are attenuated by the decrease in the number of molecular vibrations in which rapid and convenient detection of tryptophan amino acid could be performed up to a concentration of 10-7 M using the silver plasmonic substrates. In addition, by calibration, the concentration of the amino acid could be determined using silver plasmonic substrates and Raman spectroscopy, which could lead to the development of nano-sensors.

Keywords

References

[1]. M. Friedman, Analysis, Nutrition, and Health Benefits of Tryptophan. International Journal Tryptophan Research. 2018; 11: 1178646918802282.
[2]. A. Kandakkathara, I. Utkin, R. Fedosejevs. Surface-enhanced raman scattering (SERS) detection of low concentrations of tryptophan amino acid in silver colloid. Applied Spectroscopy. 2011; 65(5): 507-513.
[3]. F. Madzharova, H. Zsuzsanna, K. Janina. Surface Enhanced Hyper-Raman Scattering of the Amino Acids Tryptophan, Histidine, Phenylalanine, and Tyrosine. The Journal of Physical Chemistry C. 2017; 121(2): 1235-1242.
[4]. E.L. Twomey, E.R. Naughten, V.B. Donoghue Ryan. Neuroimaging findings in glutaric aciduria type 1. Pediatric Radiology. 2003; 33: 823-830.
[5]. J. Patsias, E.J. Papadopoulou-Mourkidou. Rapid method for the analysis of a variety of chemical classes of pesticides in surface and ground waters by off-line solid-phase extraction and gas chromatography-ion trap mass spectrometry. Journal of Chromatography. 1998; 740: 83-98.
[6]. C. Goncalves, M.F. Alpendurada. Solid-phase micro-extraction–gaschromatography–(tandem) mass spectrometry as a tool for pesticide residue analysis in water samples at high sensitivity and selectivity with confirmation capabilities. Journal of Chromatography. 2004; 1020: 239-250.
[7]. S. Zavatski, N. Khinevich, K. Girel, S. Redko, N. Kovalchuk, I. Komissarov, V. Lukashevich, I. Semak, K. Mamatkulov, V. Vorobyeva, G. Arzumanyan, H. Bandarenka. Surface Enhanced Raman Spectroscopy of Lactoferrin Adsorbed on Silvered Porous Silicon Covered with Graphene. Biosensors. 2019; 9(1): 34.
[8]. S.G. Skoulika, C.A. Georgiou.Univariate and Multivariate Calibration for the Quantitative Determination of Methyl-parathion in Pesticide Formulations by FT-Raman Spectroscopy. Applied Spectroscopy. 2000; 54: 747-752.
[9]. R.Y. Sato-Berru, J. Medina-Valtierra, C. Medina Gutierrez, C. Frausto-Reyes. Quantitative NIR–Raman analysis of methyl-parathion pesticide microdroplets on aluminum substrates. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy.2004; 60: 2231-2234.
[10]. M. Alak-Ala, T. Vo-Dinh. Surface-enhanced Raman spectrometry of organo phosphorus chemical agents. ACS Publication Analytical Chemistry.1987; 59: 2149-2153.
[11]. A.N. Ivanov, G.A. Evtyugin, K.Z. Brainina, G.K. Budnikov, L.E. Stenina. Cholinesterase Sensors Based on Thick-Film Graphite Electrodes for the Flow-Injection Determination of Organophosphorus Pesticides. Journal of Analytical Chemistry. 2002; 57: 1042-1048.
[12]. T. Alizadeh. HighSelective Parathion Voltammetric Sensor Development by Using an Acrylic Based Molecularly Imprinted Polymer-Carbon Paste Electrode. Electroanalysis. 2009; 21: 1490-1498.
[13]. N. Duan, B. Chang, H. Zhang, Z. Wang, S. Wu. Salmonella typhimurium detection using a surface-enhanced Raman scattering-based aptasensor. International Journal Food Microbiology. 2016; 218: 38-43.
[14]. L.R. Wang, Y. Fang. IR-SERS study and theoretical analogue on the adsorption behavior of pyridine carboxylic acid on silver nanoparticles. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 2006; 63: 614-618.
[15]. B. Ren, G.K. Liu, X.B. Lian, Z.L. Yang, Z.Q. Tian. Raman spectroscopy on transition metals. Analytical and bioanalytical chemistry. 2007; 388: 29-45.
[16]. C. Matricardi, C. Hanske, J.L. Garcia-Pomar, J. Langer, A. Mihi, L.M. Liz-Marzan. Gold Nanoparticle Plasmonic Superlattices as Surface-Enhanced Raman Spectroscopy Substrates. ACS Nano. 2018; vol. 12: 8531-8539.
[17]. K.Q. Lin, J. Yi, S. Hu, B.J. Liu, J.Y. Liu, X. Wang, B. Ren . Size effect on SERS of gold nanorods demonstrated via single nanoparticle spectroscopy. The Journal of Physical Chemistry C. 2016; 120: 20806-20811.
[18]. D. Darya Radziuk, H. Moehwald. Prospects for plasmonic hot spots in single molecule SERS towards the chemical imaging of live cells. Journal of Physical Chemistry Chemical Physics. 2015; 17: 21072-21093.
[19]. N. Biswas, S. Kapoor, H.S. Mahal, T. Mukherjee. Adsorption of CGA on colloidal silver particles: DFT and SERS study. Chemical Physics Letters. 2007; 444: 338-345.
[20]. S.S.R. Dasary, U.S. Rai, H. Yu, Y. Anjaneyulu, M. Dubey, P. C. Ray. Gold nanoparticle based surface enhanced fluorescence for detection of organophosphorus agents. Chemical Physics Letters. 2008; 460: 187-190.
[21]. R. Botta, A. Rajanikanth, C. Bansal. Surface Enhanced Raman Scattering studies of l-amino acids adsorbedon silver nanoclusters. Chemical Physics Letters. 2015; 618: 14-19
[22]. N. Sharifi, N. Taghavinia. Silver nano-islands on glass fibers using heat segregation method. Materials. Chemistry. and. Physics. 2009; 113: 63-66.
[23]. P.K. Ngumbi, S.W. Mugo, J.M. Ngaruiya. Determination of Gold Nanoparticles Sizes via Surface Plasmon Resonance. IOSR Journal of Applied Chemistry (IOSR-JAC). 2018; 11: 25-29.
[24]. H. Tarik Baytekin, B. Baytekin, S. Huda, Z. Yavuz, B.A. Grzybowski. Mechanochemical Activation and Patterning of an Adhesive Surface toward Nanoparticle Deposition. Journal of the American Chemical Society. 2015; 137: 1726-1729.
[25]. C.F. Bohren, D.R. Huffman. Absorption and Scattering of Light by Small Particles. Wiley. New York. 1983; 306: 625.
[26]. S.Y. Ding, E.M. You, Z.Q. Tian, M. Moskovits. Electromagnetic theories of surface-enhanced Raman spectroscopy. Journal of Chemical Society Reviews. 2017; 46: 4042-4076.
[27]. H.Y. Chen, M.H. Lin, C.Y. Wang, Y.M. , Large-scale hot spot engineering for quantitative SERS at the single-molecule scale. Journal of the American Chemical Society. 2015; 42: 13698-13705.
[28]. J.H. Granger, N.E. Schlotter, A.C. Crawford, M.D. Porter. Prospects for point-of-care pathogen diagnostics using surface-enhanced Raman scattering (SERS). Chemical Society Reviews. 2016; 45: 3865-3882.
[29]. H.M. Parsons, D.R. Ekman, T.W. Collette, M.R. Viant. Spectral relative standard deviation: a practical benchmark in metabolomics. Analyst. 2009; 134: 478–485.
[30]. F. al-Taher, R. Juskelis, Y. Chen, J. Kapposso. Comprehensive Pesticide analysis in Juice Using a Combination of GC-MS and LC-MS Methods. Application note, Food Safety, Aligent Technologies. 2012; 23(6): 579-586.
[31]. H. Fang, C.X. Zhang, L. Liu, Y.M. Zhao, H.J. Xu. Recyclable three-dimensional Ag nanoparticle-decorated TiO2 nanorod arrays for surface-enhanced Raman scattering. Biosens Bioelectron. 2015; 64: 434–441.

Files

Share

How to cite

Statistics